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Combining Peptide and DNA for Protein Assay: CRIP1 Detection for Breast Cancer Staging

12 Dec 2013

Abstract excerpt

In this work, a novel method for a protein assay is proposed which uses the specific protein-binding peptide of the target protein and sequence-specific DNA to interact with the target as the capture and detection probe, respectively. Meanwhile, since the DNA sequence can be coupled with gold nanoparticles to amplify the signal readout, a sensitive and easily operated method for protein assay is developed. We...

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PMID: 24328073
DOI: 10.1021/am404506g

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