Article
Detection of HIV-1 neutralizing antibodies in a human CD4⁺/CXCR4⁺/CCR5⁺ T-lymphoblastoid cell assay system.
PloS one - 1 Jan 2013
McLinden Robert J, Labranche Celia C, Chenine Agnès-Laurence, Polonis Victoria R, Eller Michael A, Wieczorek Lindsay, Ochsenbauer Christina, Kappes John C, Perfetto Stephen, Montefiori David C, Michael Nelson L, Kim Jerome H
Abstract excerpt
Sensitive assays are needed to meaningfully assess low levels of neutralizing antibodies (NAbs) that may be important for protection against the acquisition of HIV-1 infection in vaccine recipients. The current assay of choice uses a non-lymphoid cell line (TZM-bl) that may lack sensitivity owing to over expression of CD4 and CCR5. We used transfection of a human CD4+/CXCR4+/α4β7+ T-lymphoblastoid cell line...
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