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Article

Exploring intermolecular interactions of a substrate binding protein using a riboswitch-based sensor.

Chemistry & biology - 19 Dec 2013

Fowler Casey C, Sugiman-Marangos Seiji, Junop Murray S, Brown Eric D, Li Yingfu

Abstract excerpt

The study of biological transporters can be hampered by a dearth of methodology for tracking their activity within cells. Here, we present a means of monitoring the function of transport machinery within bacteria, exploiting a genetically encoded riboswitch-based sensor to detect the accumulation of the substrate in the cytoplasm. This method was used to investigate the model ABC transporter BtuC2D2F, which...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 24290881
DOI: 10.1016/j.chembiol.2013.10.014

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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