Article
Cryopreserved mouse pancreatic acinar cells from long-term explant outgrowth cultures maintain their secretory phenotype after thawing.
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] - 1 Jan 2000
Bläuer Merja, Sand Juhani, Laukkarinen Johanna
Abstract excerpt
BACKGROUND/OBJECTIVES: We recently reported an explant outgrowth culture method for obtaining functionally competent mouse pancreatic acinar cells for long-term in vitro purposes. The aim of the present study was to explore the possibility of cryostoring these cells without loss of functional differentiation. METHODS: Acinar cells prepared by the explant outgrowth method were cryopreserved using a DMSO-based...
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