Article
A highly facile and specific assay for cancer-causing isocitrate dehydrogenase mutant using 13C4-labeled α-ketoglutarate and heteronuclear NMR.
Analytical chemistry - 17 Dec 2013
Wen He, Yun Taeho, Xu Wen Jun, Choi Seung Hong, Kim Hyeonjin, Park Chul-Kee, Lee Se-Hoon, Park Sung-woo, Lee Sang Kook, Park Sunghyouk
Abstract excerpt
Isocitrate dehydrogenase mutations with neomorphic activity of converting α-ketoglutarate to 2-hydroxyglutarate have been found in many types of cancers. We report an NMR-based assay specific for the mutant using (13)C4-labeled α-ketoglutarate. It can be done in a complex mixture without extraction, give time-dependent absolute quantitation, and be applied to enzyme inhibition studies. Its merits over...
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