Article
Protein-mediated chromosome pairing of repetitive arrays.
Journal of molecular biology - 6 Feb 2014
Mirkin Ekaterina V, Chang Frederick S, Kleckner Nancy
Abstract excerpt
Chromosomally integrated arrays of lacO and tetO operator sites visualized by LacI and TetR repressor proteins fused with GFP (green fluorescent protein) (or other fluorescent proteins) are widely used to monitor the behavior of chromosomal loci in various systems. However, insertion of such arrays and expression of the corresponding proteins is known to perturb genomic architecture. In several cases,...
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