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Article

Engineering the glucansucrase GTFR enzyme reaction and glycosidic bond specificity: toward tailor-made polymer and oligosaccharide products.

Biochemistry - 24 Jun 2008

Hellmuth Hendrik, Wittrock Sabine, Kralj Slavko, Dijkhuizen Lubbert, Hofer Bernd, Seibel Jürgen

Abstract excerpt

Two long-standing questions about glucansucrases (EC 2.4.1.5) are how they control oligosaccharide versus polysaccharide synthesis and how they direct their glycosidic linkage specificity. This information is required for the production of tailor-made saccharides. Mutagenesis promises to be an effective tool for enzyme engineering approaches for altering the regioselectivity and acceptor substrate specificity....

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PMID: 18512955
DOI: 10.1021/bi800563r

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