Article
Preservation of protein dynamics in dihydrofolate reductase evolution.
The Journal of biological chemistry - 13 Dec 2013
Francis Kevin, Stojkovic Vanja, Kohen Amnon
Abstract excerpt
The hydride transfer reaction catalyzed by dihydrofolate reductase (DHFR) is a model for examining how protein dynamics contribute to enzymatic function. The relationship between functional motions and enzyme evolution has attracted significant attention. Recent studies on N23PP Escherichia coli DHFR (ecDHFR) mutant, designed to resemble parts of the human enzyme, indicated a reduced single turnover rate. NMR...
Topics
- Amino Acid Sequence
- Animals
- Biocatalysis
- Catalytic Domain
- Dogs
- Escherichia coli
- Evolution, Molecular
- Humans
- Kinetics
- Mice
- Models, Molecular
- Molecular Sequence Annotation
- Molecular Sequence Data
- Movement
- Mutation
- Temperature
- Tetrahydrofolate Dehydrogenase