Article
Transport of 2-methyl-4-amino-5-hydroxymethylpyrimidine in Saccharomyces cerevisiae.
Biochimica et biophysica acta - 28 Feb 1990
Iwashima A, Kawasaki Y, Kimura Y
Abstract excerpt
The transport of 2-methyl-4-amino-5-hydroxymethylpyrimidine (hydroxymethylpyrimidine) was studied in resting cells of Saccharomyces cerevisiae. Hydroxymethylpyrimidine uptake was an energy- and temperature-dependent process which has an optimal pH at 4.5. The apparent Km for hydroxymethylpyrimidine uptake was 0.37 microM, and the uptake was inhibited by 2-methyl-4-amino-5-aminomethylpyrimidine, thiamin and...
Topics
- Biological Transport, Active
- Hydrogen-Ion Concentration
- Kinetics
- Mutation
- Pyrimidines
- Pyrithiamine
- Saccharomyces cerevisiae
- Temperature
- Thiamine