Article
Regulation of THI4 (MOL1), a thiamine-biosynthetic gene of Saccharomyces cerevisiae.
Yeast (Chichester, England) - 1 Apr 1994
Praekelt U M, Byrne K L, Meacock P A
Abstract excerpt
THI4, a Saccharomyces cerevisiae gene originally identified as a result of transient expression in molasses medium and named MOL1 is regulated by thiamine. Using a THI4 promoter-lacZ fusion on a centromeric yeast vector, we have shown that the THI4 is completely repressed throughout batch culture by thiamine at a concentration around 1 microM, but shows high level constitutive expression in thiamine-free medium....
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