Article
Isolation and characterization of the THI6 gene encoding a bifunctional thiamin-phosphate pyrophosphorylase/hydroxyethylthiazole kinase from Saccharomyces cerevisiae.
The Journal of biological chemistry - 2 Dec 1994
Nosaka K, Nishimura H, Kawasaki Y, Tsujihara T, Iwashima A
Abstract excerpt
Thiamin-phosphate pyrophosphorylase (TMP-PPase; EC 2.5.1.3) involved in de novo synthesis of thiamin in Saccharomyces cerevisiae is a bifunctional enzyme with 4-methyl-5-beta-hydroxyethylthiazole kinase (Th-kinase; EC 2.7.1.50) activity, which is an octamer of identical 60-kDa subunits (Kawasaki,...
Topics
- Alkyl and Aryl Transferases
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA Primers
- Gene Library
- Genes, Fungal
- Genotype
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phosphotransferases (Alcohol Group Acceptor)
- Plasmids
- Point Mutation
- Polymerase Chain Reaction
- Recombinant Proteins
- Restriction Mapping
- Saccharomyces cerevisiae