Article
Specific characterization of substrate and inhibitor binding sites of a glycosyl hydrolase family 11 xylanase from Aspergillus niger.
The Journal of biological chemistry - 15 Nov 2002
Tahir Tariq A, Berrin Jean-Guy, Flatman Ruth, Roussel Alain, Roepstorff Peter, Williamson Gary, Juge Nathalie
Abstract excerpt
The importance of aromatic and charged residues at the surface of the active site of a family 11 xylanase from Aspergillus niger was evaluated using site-directed mutagenesis. Ten mutant proteins were heterologously produced in Pichia pastoris, and their biochemical properties and kinetic paramet...
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