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Article

Specific characterization of substrate and inhibitor binding sites of a glycosyl hydrolase family 11 xylanase from Aspergillus niger.

The Journal of biological chemistry - 15 Nov 2002

Tahir Tariq A, Berrin Jean-Guy, Flatman Ruth, Roussel Alain, Roepstorff Peter, Williamson Gary, Juge Nathalie

Abstract excerpt

The importance of aromatic and charged residues at the surface of the active site of a family 11 xylanase from Aspergillus niger was evaluated using site-directed mutagenesis. Ten mutant proteins were heterologously produced in Pichia pastoris, and their biochemical properties and kinetic parameters were determined. The specific activity of the Y6A, Y10A, Y89A, Y164A, and W172A mutant enzymes was drastically...

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Identifiers

PMID: 12207016
DOI: 10.1074/jbc.M205657200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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