Article
Structural elements to convert Escherichia coli alpha-xylosidase (YicI) into alpha-glucosidase.
FEBS letters - 15 May 2006
Okuyama Masayuki, Kaneko Akira, Mori Haruhide, Chiba Seiya, Kimura Atsuo
Abstract excerpt
Escherichia coli YicI, a member of glycoside hydrolase family (GH) 31, is an alpha-xylosidase, although its amino-acid sequence displays approximately 30% identity with alpha-glucosidases. By comparing the amino-acid sequence of GH 31 enzymes and through structural comparison of the (beta/alpha)(8) barrels of GH 27 and GH 31 enzymes, the amino acids Phe277, Cys307, Phe308, Trp345, Lys414, and beta-->alpha loop 1...
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