Article
Human epidermal Langerhans cells undergo profound morphologic and phenotypical changes during in vitro culture.
The Journal of investigative dermatology - 1 Feb 1990
Teunissen M B, Wormmeester J, Krieg S R, Peters P J, Vogels I M, Kapsenberg M L, Bos J D
Abstract excerpt
Morphology, phenotype, and enzyme activity of highly enriched (80%) unlabeled human epidermal Langerhans cells (LC) have been studied, with emphasis on changes during a short-term culture of three days in vitro. All freshly isolated LC contained Birbeck granules and expressed high levels of CD1a, CD1c, and MHC class II molecules HLA-DR, -DP, and -DQ. They have a weak to moderate expression of RFD1, C3biR, Fc...
Topics
- Animals
- Antibodies
- Cell Separation
- Cells, Cultured
- Dendritic Cells
- HLA-DP Antigens
- HLA-DQ Antigens
- HLA-DR Antigens
- Histocytochemistry
- Humans
- Langerhans Cells