Functional analysis of the Shiga toxin and Shiga-like toxin type II variant binding subunits by using site-directed mutagenesis.

doi:10.1128/jb.172.2.653-658.1990

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Article

Functional analysis of the Shiga toxin and Shiga-like toxin type II variant binding subunits by using site-directed mutagenesis.

Journal of bacteriology - 1 Feb 1990

Jackson M P, Wadolkowski E A, Weinstein D L, Holmes R K, O'Brien A D

Abstract excerpt

The B subunit of Shiga toxin and the Shiga-like toxins (SLTs) mediates receptor binding, cytotoxic specificity, and extracellular localization of the holotoxin. While the functional receptor for Shiga toxin, SLT type I (SLT-I), and SLT-II is the glycolipid designated Gb3, SLT-II variant (SLT-IIv) may use a different glycolipid receptor. To identify the domains responsible for receptor binding, localization in...

Topics

Amino Acid Sequence
Animals
Bacterial Toxins
Base Sequence
Binding Sites
Cell Line
Cell Survival
Genetic Variation
HeLa Cells
Humans
Macromolecular Substances

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Oligonucleotide Probes

Operon

Plasmids

Receptors, Cell Surface

Receptors, Immunologic

Source

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Identifiers

PMID: 2404947
DOI: 10.1128/jb.172.2.653-658.1990
PMCID: PMC208490

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2404947-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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