Article
Precise dissection of an Escherichia coli O157:H7 outbreak by single nucleotide polymorphism analysis.
Journal of clinical microbiology - 1 Dec 2013
Turabelidze George, Lawrence Steven J, Gao Hongyu, Sodergren Erica, Weinstock George M, Abubucker Sahar, Wylie Todd, Mitreva Makedonka, Shaikh Nurmohammad, Gautom Romesh, Tarr Phillip I
Abstract excerpt
The current pathogen-typing methods have suboptimal sensitivities and specificities. DNA sequencing offers an opportunity to type pathogens with greater degrees of discrimination using single nucleotide polymorphisms (SNPs) than with pulsed-field gel electrophoresis (PFGE) and other methodologies. In a recent cluster of Escherichia coli O157:H7 infections attributed to salad bar exposures and romaine lettuce, a...
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