Article
Interrogating genomic diversity of E. coli O157:H7 using DNA tiling arrays.
Forensic science international - 24 May 2007
Jackson Scott A, Mammel Mark K, Patel Isha R, Mays Tammy, Albert Thomas J, LeClerc J Eugene, Cebula Thomas A
Abstract excerpt
Here, we describe a novel microarray-based approach for investigating the genomic diversity of Escherichia coli O157:H7 in a semi-high throughput manner using a high density, oligonucleotide-based microarray. This microarray, designed to detect polymorphisms at each of 60,000 base-pair (bp) positions within an E. coli genome, is composed of overlapping 29-mer oligonucleotides specific for 60 equally spaced,...
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