Article
Overexpression of wild type and SeCys/Cys mutant of human thioredoxin reductase in E. coli: the role of selenocysteine in the catalytic activity.
Free radical biology & medicine - 1 Jan 2001
Bar-Noy S, Gorlatov S N, Stadtman T C
Abstract excerpt
In contrast to Escherichia coli and yeast thioredoxin reductases, the human placental enzyme contains an additional redox center consisting of a cysteine-selenocysteine pair that precedes the C-terminal glycine residue. This reactive selenocysteine-containing center imbues the enzyme with its unusually wide substrate specificity. For expression of the human gene in E. coli, the sequence corresponding to the SECIS...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.