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Article

Total Protein Analysis as a Reliable Loading Control for Quantitative Fluorescent Western Blotting

30 Aug 2013

Abstract excerpt

Western blotting has been a key technique for determining the relative expression of proteins within complex biological samples since the first publications in 1979. Recent developments in sensitive fluorescent labels, with truly quantifiable linear ranges and greater limits of detection, have allowed biologists to probe tissue specific pathways and processes with higher resolution than ever before. However, the...

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PMID: 24023619
DOI: 10.1371/journal.pone.0072457

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