Expression and characterization of a codon-optimized blood coagulation factor VIII.

doi:10.1111/jth.13632

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Article

Expression and characterization of a codon-optimized blood coagulation factor VIII.

Journal of thrombosis and haemostasis : JTH - 1 Apr 2017

Shestopal S A, Hao J-J, Karnaukhova E, Liang Y, Ovanesov M V, Lin M, Kurasawa J H, Lee T K, Mcvey J H, Sarafanov A G

Abstract excerpt

Essentials Recombinant factor VIII (FVIII) is known to be expressed at a low level in cell culture. To increase expression, we used codon-optimization of a B-domain deleted FVIII (BDD-FVIII). This resulted in 7-fold increase of the expression level in cell culture. The biochemical properties of codon-optimized BDD-FVIII were similar to the wild-type protein. SUMMARY: Background Production of recombinant factor...

Topics

Animals
CHO Cells
Cell Line
Codon
Cricetinae
Cricetulus
DNA, Complementary
Factor VIII
Genetic Vectors
Glycosylation
Humans
Lentivirus
Mutation
Peptide Fragments

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Engineering

Protein Structure, Secondary

Structure-Activity Relationship

Tyrosine

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Open in PubMed

Identifiers

PMID: 28109042
DOI: 10.1111/jth.13632

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