Immunoblotting of human red cell membranes: detection of glycophorin B with anti-S and anti-s antibodies.

Abstract excerpt

Human anti-S and anti-s eluates bound to glycophorin B on immunoblots from membranes of S+ and s+ red cells, respectively. Eluates of human anti-S were more efficiently prepared from sensitized trypsin-treated cells than from sensitized untreated cells. The results of immunoblotting membranes from enzyme-treated cells confirmed the serological findings: S and s antigens were not affected by treatment with trypsin...

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