Article
Structure-based protein engineering efforts with a monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties.
Protein engineering, design & selection : PEDS - 1 Apr 2008
Alahuhta Markus, Salin Mikko, Casteleijn Marco G, Kemmer Christian, El-Sayed Ibrahim, Augustyns Koen, Neubauer Peter, Wierenga Rik K
Abstract excerpt
A monomeric variant of triosephosphate isomerase (TIM) with a new engineered binding groove has been characterized further. In this variant (ml8bTIM), the phosphate binding loop had been shortened, causing the binding site to be much more extended. Here, it is reported that in the V233A variant of ml8bTIM (A-TIM), three important properties of the wild-type TIM active site have been restored: (i) the structural...
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