Article
Rapid genotyping of three polymorphisms in the LBP gene using a triplex pyrosequencing approach.
Journal of microbiological methods - 1 Sept 2013
Zeng Ling, Yu Jing, Gu Wei, Zhang An-qiang, Yue Cai-li, Chen Ke-hong, Jiang Jian-xin
Abstract excerpt
We described a triplex polymerase chain reaction (PCR) and triplex pyrosequencing assay which allowed a simultaneous determination of three tag single nuleotide polymorphisms (tag SNPs) in the lipopolysaccharide-binding protein (LBP) gene: rs1780623, rs11536972 and rs2232618. This method enables a fast and cost-effective genotyping and a simultaneous determination of the three tag SNPs.
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