Article
Identifying critical unrecognized sugar-protein interactions in GH10 xylanases from Geobacillus stearothermophilus using STD NMR.
The FEBS journal - 1 Sept 2013
Balazs Yael S, Lisitsin Elina, Carmiel Oshrat, Shoham Gil, Shoham Yuval, Schmidt Asher
Abstract excerpt
(1)H solution NMR spectroscopy is used synergistically with 3D crystallographic structures to map experimentally significant hydrophobic interactions upon substrate binding in solution under thermodynamic equilibrium. Using saturation transfer difference spectroscopy (STD NMR), a comparison is made between wild-type xylanase XT6 and its acid/base catalytic mutant E159Q--a non-active, single-heteroatom alteration...
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