Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts

5 Jul 2013

Abstract excerpt

Tissues and cell lines derived from an individual with disease are ideal sources to study disease-related cellular phenotypes. Patient-derived fibroblasts in this protocol have been successfully used in the derivation of induced pluripotent stem cells to model disease(1). Early passages of these fibroblasts can also be used for cell-based functional assays to study specific disease pathways, mechanisms(2) and...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biomedical Ethics and Regulation
Share to Pluripotent Stem Cells Research
Share to Tissue Engineering and Regenerative Medicine

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 23852182
DOI: 10.3791/3779

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 3 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Skin Punch Biopsy Explant Culture for Derivation of Primary Human FibroblastsPMID 23852182Read ↗

Long-term culture of skin biopsies: Maintenance of fibroblast production and competency of reprogrammingSemantic proximity Read ↗

A Comparative View on Human Somatic Cell Sources for iPSC GenerationSemantic proximity Read ↗

Isolation and culture of human primary keratinocytes—a methods reviewSemantic proximity Read ↗

Isolation of five different primary cell types from a single sample of human skinSemantic proximity Read ↗

Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cellsSemantic proximity Read ↗

Isolation and feeder-free primary culture of four cell types from a single human skin sampleSemantic proximity Read ↗

Viral-free generation and characterization of a human induced pluripotent stem cell line from dermal fibroblastsSemantic proximity Read ↗

Minimizing time in culture: A prototypic autologous manufacturing workflow for monoclonal iPSC lines within seven weeksSemantic proximity Read ↗

An Optimized Method to Isolate Human Fibroblasts from Tissue for Ex Vivo AnalysisSemantic proximity Read ↗

Enhanced Isolation of Fibroblasts from Human Skin ExplantsSemantic proximity Read ↗

Primary Skin Fibroblasts as a Model of Parkinson's DiseaseSemantic proximity Read ↗

A systematic comparison of fibroblasts derived from postmortem human dura mater versus dermal epithelium for neurodegenerative disease modelingSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.