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Isolation of five different primary cell types from a single sample of human skin

10 May 2022

Abstract excerpt

We have developed a technique to isolate primary keratinocytes, melanocytes, fibroblasts, preadipocytes, and microvascular endothelial cells from an individual sample of human skin. The protocol describes step-by-step instructions for processing, cells isolation, and culture of neonatal foreskin, with adaptation for more demanding adult tissues. The availability of multiple isogenic cell types derived from...

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PMID: 35586317
DOI: 10.1016/j.xpro.2022.101378

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