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Isolation and feeder-free primary culture of four cell types from a single human skin sample

10 Feb 2022

Abstract excerpt

Four types of primary cells-dermal fibroblasts, dermal microvascular endothelial cells, epidermal keratinocytes, and epidermal melanocytes-can be isolated simultaneously from a single human skin sample, without the use of xenogeneic murine feeder cells. This protocol describes the procedures for isolation of these cells from adult full-thickness skin obtained from surgical discard tissue. The cells isolated using...

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PMID: 35199036
DOI: 10.1016/j.xpro.2022.101172

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