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Enhanced Isolation of Fibroblasts from Human Skin Explants

1 Oct 2012

Abstract excerpt

Here we describe a method for growing fibroblasts from human skin explants that increases the number of cells obtained by up to two orders of magnitude, thus increasing the amount of material available for research and diagnostic purposes and potentially for cell-based therapies. Explants can be transferred sequentially up to 80 times, if required, at which point the explants appear to be completely depleted of...

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PMID: 23046507
DOI: 10.2144/0000113939

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