Article
Multiple pqqA genes respond differently to environment and one contributes dominantly to pyrroloquinoline quinone synthesis.
Journal of basic microbiology - 1 Mar 2015
Ge Xin, Wang Wenxi, Du Baohua, Wang Jianhua, Xiong Xianghua, Zhang Weicai
Abstract excerpt
Pyrroloquinoline quinone is the third redox cofactor after nicotinamide and flavin in bacteria, and its biosynthesis pathway comprise five steps initiated from a precursor peptide PqqA coded by pqqA gene. Methylovorus sp. MP688 is equipped with five copies of pqqA genes. Herein, the transcription of pqqA genes under different conditions by real-time quantitative PCR and β-galactosidase reporter genes are...
Topics
- Bacterial Proteins
- Escherichia coli
- Gene Deletion
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Hydrogen-Ion Concentration
- Methylophilaceae
- Multigene Family
- Mutation
- PQQ Cofactor
- Promoter Regions, Genetic
- Real-Time Polymerase Chain Reaction
- Sequence Analysis, DNA