Article
Synthesis of pyrroloquinoline quinone in vivo and in vitro and detection of an intermediate in the biosynthetic pathway.
Journal of bacteriology - 1 Sept 1995
Velterop J S, Sellink E, Meulenberg J J, David S, Bulder I, Postma P W
Abstract excerpt
In Klebsiella pneumoniae, six genes, constituting the pqqABCDEF operon, which are required for the synthesis of the cofactor pyrroloquinoline quinone (PQQ) have been identified. The role of each of these K. pneumoniae Pqq proteins was examined by expression of the cloned pqq genes in Escherichia coli, which cannot synthesize PQQ. All six pqq genes were required for PQQ biosynthesis and excretion into the medium...
Topics
- Aerobiosis
- Anaerobiosis
- Bacterial Proteins
- Coenzymes
- Escherichia coli
- Genes, Bacterial
- Genetic Complementation Test
- Glucose Dehydrogenases
- Klebsiella pneumoniae
- Mutation
- Operon