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Article

Effect of Mutation and Substrate Binding on the Stability of Cytochrome P450BM3 Variants.

Biochemistry - 28 Jun 2016

Geronimo Inacrist, Denning Catherine A, Rogers W Eric, Othman Thaer, Huxford Tom, Heidary David K, Glazer Edith C, Payne Christina M

Abstract excerpt

Cytochrome P450BM3 is a heme-containing enzyme from Bacillus megaterium that exhibits high monooxygenase activity and has a self-sufficient electron transfer system in the full-length enzyme. Its potential synthetic applications drive protein engineering efforts to produce variants capable of oxidizing nonnative substrates such as pharmaceuticals and aromatic pollutants. However, promiscuous P450BM3 mutants often...

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Identifiers

PMID: 27267136
DOI: 10.1021/acs.biochem.6b00183
PMCID: PMC7422958

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Source receipt: pubmed, retrieved 7 Sept 2026.

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Effect of Mutation and Substrate Binding on the Stability of Cytochrome P450BM3 Variants.PMID 27267136Read ↗

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