Article
Acute inactivation of the Aspergillus nidulans Golgi membrane fusion machinery: correlation of apical extension arrest and tip swelling with cisternal disorganization.
Molecular microbiology - 1 Jul 2013
Pinar Mario, Pantazopoulou Areti, Arst Herbert N, Peñalva Miguel A
Abstract excerpt
The mechanisms governing traffic across the Golgi are incompletely understood. We studied, by live-cell microscopy, the consequences of disorganizing the Aspergillus nidulans Golgi, using an extended set of fluorescent protein markers to resolve early from late cisternae. The early Golgi syntaxin SedV(Sed) (5) and the RabO(Rab) (1) regulatory GTPase play essential roles in secretion, cooperating in the ER-Golgi...
Topics
- Aspergillus nidulans
- Fungal Proteins
- GTP Phosphohydrolases
- Golgi Apparatus
- Green Fluorescent Proteins
- Hyphae
- Intracellular Membranes
- Membrane Fusion
- Microscopy, Confocal
- Microscopy, Fluorescence