Article
Superresolution and pulse-chase imaging reveal the role of vesicle transport in polar growth of fungal cells.
Science advances - 1 Jan 2018
Zhou Lu, Evangelinos Minoas, Wernet Valentin, Eckert Antonia F, Ishitsuka Yuji, Fischer Reinhard, Nienhaus G Ulrich, Takeshita Norio
Abstract excerpt
Polarized growth of filamentous fungi requires continuous transport of biomolecules to the hyphal tip. To this end, construction materials are packaged in vesicles and transported by motor proteins along microtubules and actin filaments. We have studied these processes with quantitative superresolution localization microscopy of live Aspergillus nidulans cells expressing the photoconvertible protein mEosFPthermo...
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