Article
Site-directed mutagenesis reveals a liver transcription factor essential for the albumin transcriptional enhancer.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jul 1990
Zaret K S, Liu J K, DiPersio C M
Abstract excerpt
A method based on the polymerase chain reaction is described for constructing a clustered set of basepair changes, deletions, or insertions at any site on a DNA fragment. Advantages of the procedure are that virtually every product has the desired sequence alteration and that only a single round...
Topics
- Animals
- Base Sequence
- Cell Nucleus
- Deoxyribonuclease I
- Enhancer Elements, Genetic
- Liver
- Mice
- Molecular Sequence Data
- Mutation
- Nuclear Proteins
- Oligonucleotide Probes
- Polymerase Chain Reaction