Article
Site-directed mutagenesis reveals a liver transcription factor essential for the albumin transcriptional enhancer.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jul 1990
Zaret K S, Liu J K, DiPersio C M
Abstract excerpt
A method based on the polymerase chain reaction is described for constructing a clustered set of basepair changes, deletions, or insertions at any site on a DNA fragment. Advantages of the procedure are that virtually every product has the desired sequence alteration and that only a single round of polymerase chain reaction is required. We used this method to demonstrate that the binding of a specific liver...
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