Article

AAV-mediated genome editing is influenced by the formation of R-loops

2024-05-09

Abstract excerpt

Recombinant adeno-associated viral vectors (rAAV) hold an intrinsic ability to stimulate homologous recombination (AAV-HR) and are the most used in clinical settings for in vivo gene therapy. However, rAAVs also integrate throughout the genome. Here, we describe DNA-RNA immunoprecipitation sequencing (DRIP-seq) in murine HEPA1-6 hepatoma cells and whole murine liver to establish the similarities and differences i...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 8cb91d13-01e1-5abc-9bda-df9743123f82
DOI: 10.1101/2024.05.07.592855

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

AAV-mediated genome editing is influenced by the formation of R-loopsDOI 10.1101/2024.05.07.592855Read

Genome-wide assays to characterize rAAV integration into human genomic DNA in vivoSemantic proximity Read

Identification of a robust promoter in mouse and human hepatocytes by in vivo biopanning of a barcoded AAV librarySemantic proximity Read

Molecular characterization of precise in vivo targeted gene integration in human cells using AAVHSC15.Semantic proximity Read

Single-Cell Transcriptome Analysis of Mouse Liver Cell-Specific Tropism and Transcriptional Dysregulation Following Intravenous Administration of AAVrh.10 VectorsSemantic proximity Read

Genome-scale analysis of cellular restriction factors that inhibit transgene expression from adeno-associated virus vectorsSemantic proximity Read

Safe and effective liver-directed AAV-mediated homology-independent targeted integration in mouse models of inherited diseasesSemantic proximity Read

Inherent hepatocytic heterogeneity determines expression and retention of edited F9 alleles post-AAV/CRISPR infusion.Semantic proximity Read

Variability in R-loops levels based on IHC detectionSemantic proximity Read

A single amino acid variant in the variable region I of AAV capsid confers liver detargetingSemantic proximity Read

Genetic manipulation using hepatocyte-targeting adeno-associated viral vectors has minimal off-target effectsSemantic proximity Read

Genome-wide CRISPR screenings identified SMCHD1 as a host-restricting factor for AAV transductionSemantic proximity Read

Isolation of Recombinant Adeno-Associated Virus Vector-Cellular DNA Junctions from Mouse LiverSemantic proximity Read