Article
Rapid electrochemical screening of NAD-dependent dehydrogenases in a 96-well format.
Chemical communications (Cambridge, England) - 28 Jun 2013
Abdellaoui Sofiène, Bekhouche Mourad, Noiriel Alexandre, Henkens Robert, Bonaventura Celia, Blum Loïc J, Doumèche Bastien
Abstract excerpt
The electrochemical detection of dehydrogenase activity in crude cell lysates is performed simultaneously using 96 carbon electrodes modified with electrografted phenazines. The method is applied to the screening of a library of formate dehydrogenase mutants obtained by directed evolution.
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