Article
A next-generation sequencing method for genotyping-by-sequencing of highly heterozygous autotetraploid potato.
PloS one - 1 Jan 2013
Uitdewilligen Jan G A M L, Wolters Anne-Marie A, D'hoop Bjorn B, Borm Theo J A, Visser Richard G F, van Eck Herman J
Abstract excerpt
Assessment of genomic DNA sequence variation and genotype calling in autotetraploids implies the ability to distinguish among five possible alternative allele copy number states. This study demonstrates the accuracy of genotyping-by-sequencing (GBS) of a large collection of autotetraploid potato cultivars using next-generation sequencing. It is still costly to reach sufficient read depths on a genome wide scale,...
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