Recombination between directly repeated origins of conjugative transfer cloned in M13 bacteriophage DNA models ligation of the transferred plasmid strand.

doi:10.1093/nar/18.12.3579

Back to search

Article

Recombination between directly repeated origins of conjugative transfer cloned in M13 bacteriophage DNA models ligation of the transferred plasmid strand.

Nucleic acids research - 25 Jun 1990

Barlett M M, Erickson M J, Meyer R J

Abstract excerpt

When two, directly-repeated copies of the origin of transfer (oriT) of the conjugatively mobilizable, broad host-range plasmid R1162 are cloned into bacteriophage M13mp9 DNA, they undergo recombination in the presence of one of the R1162-encoded proteins required for mobilization [Meyer, R. (1989...

Topics

Bacteriophages
Base Sequence
Cloning, Molecular
Conjugation, Genetic
DNA, Viral
Molecular Sequence Data
Mutation
Plasmids
Recombination, Genetic
Repetitive Sequences, Nucleic Acid

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.