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An Escherichia coli strain, BJ5183, that shows highly efficient conservative (two-progeny) DNA double-strand break repair of restriction breaks.

Gene - 16 Jan 2003

Takahashi Noriko, Yoshikura Hiroshi, Kobayashi Ichizo

Abstract excerpt

We examined the mode of recombination in an Escherichia coli strain, BJ5183, which has been frequently used in recovery and cloning of eukaryotic DNA. One of the important criteria in characterizing a homologous recombination mechanism is whether it produces two recombinant DNA molecules or only one recombinant DNA molecule out of two parental DNA molecules. Our previous work transferring plasmid molecules with a...

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PMID: 12559570
DOI: 10.1016/s0378-1119(02)01107-1

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