Article
27 amino acid residues can be deleted from the N-terminus of human lymphotoxin without impairment of its cytotoxic activity.
Journal of molecular recognition : JMR - 1 Apr 1990
Nishikawa S, Matsuo N, Isaka Y, Uesugi S, Miura K, Ohtsuka E, Takeda K, Konno K, Ikehara M
Abstract excerpt
In order to study the relationship between activity and structure of human lymphotoxin (hLT, 171 aa), we synthesized the gene (519 bp) for hLT and expressed it in Escherichia coli. Purification of the recombinant hLT from crude extracts was difficult because of the low level of expression of the...
Topics
- Amino Acid Sequence
- Base Sequence
- Cytotoxicity, Immunologic
- Humans
- Lymphotoxin-alpha
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Recombinant Proteins
- Structure-Activity Relationship