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Article

27 amino acid residues can be deleted from the N-terminus of human lymphotoxin without impairment of its cytotoxic activity.

Journal of molecular recognition : JMR - 1 Apr 1990

Nishikawa S, Matsuo N, Isaka Y, Uesugi S, Miura K, Ohtsuka E, Takeda K, Konno K, Ikehara M

Abstract excerpt

In order to study the relationship between activity and structure of human lymphotoxin (hLT, 171 aa), we synthesized the gene (519 bp) for hLT and expressed it in Escherichia coli. Purification of the recombinant hLT from crude extracts was difficult because of the low level of expression of the gene. To improve the yield of the recombinant protein, we prepared five truncated genes for mutant proteins in which...

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Identifiers

PMID: 2361063
DOI: 10.1002/jmr.300030207

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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