Article
[Mutation of lambdapL/pR-cI857 system for production of bacterial ghost in Escherichia coli].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Dec 2012
Dong Hongliang, Han Xian'gan, Bai Hao, He Liang, Liu Lei, Liu Rui, Chai Tongjie, Ding Chan, Liu Haiwen, Yu Shengqing
Abstract excerpt
Bacterial ghost is intact envelope of Gram-negative bacteria, which is produced by the function of the lysis gene E from bacteriophage PhiX174. The expression of the lysis gene E is usually controlled by the thermosensitive lambdapL/pR-cI857 promoter. In this study, we described a mutation (T --> C) at the ninth nucleotide of the OR2 in the lambdapR promoter of the lambdapL/pR-cI857 system by overlap PCR. The...
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