Article
Controlled expression of lysis gene E by a mutant of the promoter pL of the thermo-inducible λcI857-pL system.
Journal of applied microbiology - 1 Jun 2021
Fu L X, Gong J S, Gao B, Ji D J, Han X G, Zeng L B
Abstract excerpt
AIMS: To identify a lambda promoter pL mutant that could extend the thermal stability of the thermo-inducible λcI857-pR/pL system and to evaluate the effects of the modified system for the controlled expression of lysis gene E during the production of bacterial ghosts (BGs). METHODS AND RESULTS: The promoter pL mutant was identified by random mutagenesis and site-directed mutagenesis. The results showed that a...
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