Genetic and structural characterization of endA. A membrane-bound nuclease required for transformation of Streptococcus pneumoniae.

doi:10.1016/S0022-2836(05)80259-1

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Article

Genetic and structural characterization of endA. A membrane-bound nuclease required for transformation of Streptococcus pneumoniae.

Journal of molecular biology - 20 Jun 1990

Puyet A, Greenberg B, Lacks S A

Abstract excerpt

The endA gene encoding the membrane nuclease of Streptococcus pneumoniae, which is necessary for DNA uptake in genetic transformation, was cloned in a streptococcal vector. This was accomplished by insertional mutagenesis of the gene, cloning of the mutant allele, and substitution of the wild-typ...

Topics

Amino Acid Sequence
Bacterial Proteins
Base Sequence
Cell Membrane
Cloning, Molecular
DNA, Bacterial
Deoxyribonucleases
Endodeoxyribonucleases
Genes, Bacterial
Genetic Linkage
Membrane Proteins
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Plasmids

Restriction Mapping

Sequence Homology, Nucleic Acid

Streptococcus pneumoniae

Transformation, Bacterial

Source

Open in PubMed

Identifiers

PMID: 2359120
DOI: 10.1016/S0022-2836(05)80259-1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2359120-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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