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[Synthesis of visual rhodopsin in a cell-free translation system. II. Functional properties of recombinant rhodopsin and its mutant forms].

Bioorganicheskaia khimiia - 1 Mar 1990

Gurevich V V, Zozulia S A, Shirokova E P, Zviaga T A, Garnovskaia M N, Dumler I L, Badalov P R, Natochin M Iu, Pokrovskaia I D, Shmukler B E

Abstract excerpt

Functional expression of bovine visual rhodopsin in the cell-free translation system with cotranslational insertion of the protein into phosphatidylcholine liposomes is described. The recombinant rhodopsin has spectral and functional properties similar to those of natural rhodopsin from bovine retina. Two mutant rhodopsins with amino acid substitutions in the hydrophilic C-terminal domain were obtained using...

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PMID: 2357235

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