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Article

Impact of the 3D microenvironment on phenotype, gene expression, and EGFR inhibition of colorectal cancer cell lines.

PloS one - 1 Jan 2013

Luca Anna C, Mersch Sabrina, Deenen René, Schmidt Stephan, Messner Isabelle, Schäfer Karl-Ludwig, Baldus Stephan E, Huckenbeck Wolfgang, Piekorz Roland P, Knoefel Wolfram T, Krieg Andreas, Stoecklein Nikolas H

Abstract excerpt

Three-dimensional (3D) tumor cell cultures grown in laminin-rich-extracellular matrix (lrECM) are considered to reflect human tumors more realistic as compared to cells grown as monolayer on plastic. Here, we systematically investigated the impact of ECM on phenotype, gene expression, EGFR signaling pathway, and on EGFR inhibition in commonly used colorectal cancer (CRC) cell lines. LrECM on-top (3D) culture...

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Source

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Identifiers

PMID: 23555746
DOI: 10.1371/journal.pone.0059689
PMCID: PMC3608563

Genes

EGFR
DLD
KRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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