Article
Use of second-site suppressor mutations in Drosophila to identify components of the transcriptional machinery.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jun 1990
Mortin M A
Abstract excerpt
Isolation of second-site suppressor mutations provides a powerful method for identifying (i) genes that encode proteins that interact and (ii) domains within the interacting proteins that contact each other. Flies conditionally lethal because they carry mutations in the largest subunit of RNA polymerase II were mutagenized; ten million progeny were then screened for compensatory mutations. Eight intragenic and 10...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.