Article
DNA replication studies with coliphage 186. III. A single phage gene is required for phage 186 replication.
Journal of molecular biology - 5 Jun 1990
Sivaprasad A V, Jarvinen R, Puspurs A, Egan J B
Abstract excerpt
We have shown that the BglII to BamHI (79.6% to 95.8%) region of the coliphage 186 chromosome can direct 186-specific replication. DNA sequencing of the region revealed five presumptive genes, CP80, CP81, CP83, CP84 and CP87. Surprisingly, alleles of the previously defined replication gene, A, were localized in both CP84 and CP87. We have successfully constructed a 186 minichromosome using the single gene CP87,...
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