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Article

Cell-free N-glycosylation in Dictyostelium discoideum: analysis of wild-type and mutants defective in lipid-linked oligosaccharide biosynthesis.

Journal of cellular biochemistry - 1 May 1990

Freeze H H, Koza-Taylor P, Jones J A, Loomis W F

Abstract excerpt

N-glycosylation was measured in wild-type cell lysates of Dictyostelium discoideum and in two mutant strains that synthesize a truncated lipid-linked oligosaccharide, Man6GlcNAc2 lacking terminal mannose and glucose residues. Endogenous lipid-linked oligosaccharide (LLO) was transferred to octanoyl-Asn-[125I]Tyr-ThrNH2 by membrane fractions. About 50% of the glycopeptide product remained associated with...

Topics

Cell-Free System
Detergents
Dictyostelium
Diploidy
Glycosylation
Haploidy
Hexosyltransferases
Kinetics
Lipid Metabolism
Mannans
Membrane Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Oligosaccharides

Temperature

Transferases

Source

Identifiers

PMID: 2347875
DOI: 10.1002/jcb.240430104

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2347875-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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