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Insulin glargine production: Codon engineering in Pichia pastoris X-33 for enhanced therapeutic yield

2025-11-25

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<title>Abstract</title> <p> Aims This study aims to investigate the optimization and expression of insulin glargine, a long-acting insulin analogue, in <italic>Pichia pastoris</italic> X-33. The focus is on enhancing the production efficiency of recombinant insulin glargine by utilizing codon optimization to increase protein yield. Materials and methods The insulin glargine gene was codon-optimized to enhance...

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Literature Corpus work: 5ec9d2cd-f042-56ed-8473-341fd419321e
DOI: 10.21203/rs.3.rs-8189748/v1

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