Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A comparative study of most probable number (MPN)-PCR vs. real-time-PCR for the measurement of abundance and assessment of diversity of alkB homologous genes in soil.

Journal of microbiological methods - 1 Mar 2010

Schulz Stephan, Peréz-de-Mora Alfredo, Engel Marion, Munch Jean Charles, Schloter Michael

Abstract excerpt

The utilization of quantitative PCR (qPCR) approaches such as MPN-qPCR and real-time-qPCR for in situ assessment of functional genes yields substantial quantitative and qualitative differences. We show this by targeting the alkB gene related to biodegradation of aliphatic alkanes in a set of envi...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacteria
Share to DNA, Bacterial
Share to DNA Primers

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Dosage

Share to Genes, Bacterial

Share to Mixed Function Oxygenases

Share to Sensitivity and Specificity

Share to Soil Microbiology

Source

Identifiers

PMID: 20079768
DOI: 10.1016/j.mimet.2010.01.005

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-20079768-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A comparative study of most probable number (MPN)-PCR vs. real-time-PCR for the measurement of abundance and assessment of diversity of alkB homologous genes in soil.PMID 20079768Read ↗

Assessing the role of alkane hydroxylase genotypes in environmental samples by competitive PCR.Semantic proximity Read ↗

Abundance and diversity of n-alkane-degrading bacteria in a forest soil co-contaminated with hydrocarbons and metals: a molecular study on alkB homologous genes.Semantic proximity Read ↗

Evaluating the assignment of alkB terminal restriction fragments and sequence types to distinct bacterial taxa.Semantic proximity Read ↗

Phylogeny and diversity of alkane-degrading enzyme gene variants in the laurentian great lakes and western atlantic.Semantic proximity Read ↗

Comparison of the specificities and efficacies of primers for aromatic dioxygenase gene analysis of environmental samples.Semantic proximity Read ↗

Design of a degenerate primer pair to target a bacterial functional community: The hppd bacterial gene coding for the enzyme targeted by herbicides, a study case.Semantic proximity Read ↗

Abundance of novel and diverse tfdA-like genes, encoding putative phenoxyalkanoic acid herbicide-degrading dioxygenases, in soil.Semantic proximity Read ↗

Activity, diversity and population size of ammonia-oxidising bacteria in oil-contaminated landfarming soil.Semantic proximity Read ↗

Quantification of phnAc and nahAc in contaminated new zealand soils by competitive PCR.Semantic proximity Read ↗

Metatranscriptomics of Soil Eukaryotic Communities.Semantic proximity Read ↗

Compositional profile of α / β-hydrolase fold proteins in mangrove soil metagenomes: prevalence of epoxide hydrolases and haloalkane dehalogenases in oil-contaminated sites.Semantic proximity Read ↗

Bacterial targets as potential indicators of diesel fuel toxicity in subantarctic soils.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.